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Reagents for Molecular Microbiology.
Specializing in DNA-PCR and RNA-RT-PCR Microbial amplification primers, Fluorescent probes, Plasmids, and Controls.
CONTROLS NEEDED FOR Nucleic acid EXTRACTION and AMPLIFICATION PRIMER & PROBES
MASTER MIXES
BACTERIAL and FUNGAL IDENTIFICATION by SEQUENCING
DNA-PCR and RNA-RT-PCR assays need to control for the following:
  • Extraction of DNA or RNA
  • Amplification
  • DNA-PCR and RNA-RT inhibition
  • Reagent quality including dNTPs, buffers, Taq, and reverse transcriptase
  • Instrument function including thermal cycling and fluorescent detection.
The ANSWER:
  • T4 bacteriophage for DNA
  • Qbeta bacteriophage for RNA
Real-Time PCR and RT-PCR tests:
  • Bacteria
  • Viruses
  • Protozoa
Reagents for quantitative assays with:
  • Plasmid controls
  • Standard curves
  • Limit of Detection.
Master mixes
and
Sequencing primers
Attostar LLC
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