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Friday, January 15, 2021
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Extraction Controls DNA & RNA
Microbial Master Mixes
Sequence Identification
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Reagents for Molecular Microbiology
.
Specializing in
DNA-PCR and RNA-RT-PCR Microbial amplification primers
,
Fluorescent probes
,
Plasmids
, and
Controls
.
CONTROLS NEEDED FOR Nucleic acid EXTRACTION and AMPLIFICATION
PRIMER & PROBES
MASTER MIXES
BACTERIAL and FUNGAL IDENTIFICATION by SEQUENCING
DNA-PCR and RNA-RT-PCR assays need to control for the following:
Extraction of DNA or RNA
Amplification
DNA-PCR and RNA-RT inhibition
Reagent quality including dNTPs, buffers, Taq, and reverse transcriptase
Instrument function including thermal cycling and fluorescent detection.
The ANSWER:
T4 bacteriophage for DNA
Qbeta bacteriophage for RNA
Real-Time PCR and RT-PCR tests:
Bacteria
Viruses
Protozoa
Reagents for quantitative assays with:
Plasmid controls
Standard curves
Limit of Detection.
Master mixes
and
Sequencing primers
Attostar
LLC